Primary human hepatocytes are widely used in in vitro drug metabolism studies, where consistent cell handling is essential for obtaining reliable and reproducible results.
In this video SOP, we demonstrate our workflow for thawing cryopreserved human hepatocytes and preparing them for metabolism incubations.
The protocol covers the complete experimental workflow, including:
- Thawing cryopreserved human hepatocytes
- Cell counting and standardization
- Setting up hepatocyte incubations in a 24-well plate
- Sampling and reaction quenching
- Sample centrifugation
- Preparation of samples for LC–MS/MS analysis
The video provides a practical step-by-step reference for setting up primary human hepatocyte metabolism assays and preparing samples for downstream analytical measurements.